Additional methods, including the HCMV viremia (detection of HCMV-infected cells after rapid isolation of HCMV using fibroblasts) and quantification of HCMV DNA in plasma after digestion with deoxyribonuclease (DNAse) (to identify DNA packaged in virions), have been proposed to identify productive viral replication [3,4]. However, the HCMV viraemia test must be performed within 24 hours of sample collection and is less sensitive than molecular assays. On the other hand, the DNase test is a time-consuming and poorly standardized test that requires the use of DNAse I in the sample before extraction to distinguish free-floating DNA from the genome packaged in virions [3,4].