3.1 Detection of UL21.5 mRNA during DNAemia episodes.
Peak levels of WB and PL DNAemia in patients requiring pre-emptive
therapy or showing self-resolving transient DNAemia are shown in Table 1
and Figure 1B. UL21.5 mRNA was detected in plasma 1-2 weeks before
administration of pre-emptive therapy in all the 20 episodes of
clinically significant DNAemia (Table 1 and Figure 1A), whereas it was
undetectable in 3 of the 4 patients with transient self-resolving
DNAemia (the only positive patient showed very low UL21.5 mRNA level
below the limit of quantification). The median kinetics of WB and PL
DNAemia and of UL21.5 mRNA (in plasma) in the 20 episodes of clinically
significant DNAemia is shown in Fig 1B. UL21.5 mRNA was detected about
two weeks after WB DNAemia appearance, and, during pre-emptive therapy,
it was cleared earlier than WB DNAmia. After start of treatment, median
time to UL21.5 mRNA clearance was 16 days, whereas median time to WB
DNAemia and PL DNAemia was 36 days (Fig. 1C).