Background: Several bacterial species contaminate the white coats and mobile phones used by students in the examined department. objectives: The aim is to identify and determine the bacterial species that are polluting white coats, students’ mobile phones, and inanimate surfaces at Alsaeeda University, Thamar city, Yemen Methods: We collected a total of 183 swab samples from various inanimate surfaces within the university, including the sleeves of white coats, the keys, interior, posterior, of mobile phones, and the surfaces of chairs, stairs, and door handles. We subjected the samples to microbiological culture and biochemical tests. Results: The study reported that 176 (96.17%) of the 178 samples evaluated tested positive for bacterial contamination . The contamination rate was highest in mobile phones and lowest in lab coats. Based on the kind of isolated bacteria, Staphylococcus aureus has the highest bacterial contamination rate, while Salmonella spp. and Streptococcus pneumoniae have the lowest. The contamination rate was higher in females than in males. According to the department, the laboratory had a higher contamination rate than the pharmacy. The results of antimicrobial susceptibility patterns of organisms isolated from swab samples showed that isolates were resistant to the drugs tested. Penicillin and optochin had the highest resistance rates among gram-positive bacteria. Conclusions: This study concludes that white coats worn by medical college students typically exhibit high levels of microbial contamination. White coats and mobile phones may serve as significant vectors for the transmission of bacterial contamination.