A SARS-CoV-2, N15 strain was successfully isolated from the nasal swab of a pediatric patient after five blind passages in Vero cells. The biological properties of this isolate were investigated extensively. Cytopathic effects (CPE) were observed within 48 hours post- infection, characterized by aggregates of dead cells. The N15 isolate demonstrated the ability to replicate across various human-origin cell lines, albeit with different growth kinetics. Genetic analyses revealed that the strain belongs to the 19B lineage of SARS-CoV-2, showing 99.9% similarity to the original Wuhan 1 strain. Importantly, the N15 strain did not exhibit genetic signatures of in-vitro adaptation and possessed rare mutations N709S in the spike protein and T11M in the E protein. Drug testing indicated that Remdesivir, Molnupiravir, and Nirmatrelvir effectively inhibited this isolate at varying concentrations. The N15 isolate may serve as valuable material for comparative studies with currently circulating SARS-CoV-2 variants.