Currently, research on the combined application of straw-made charcoal and organic fertilizer made from livestock and poultry manure is not extensive, despite their positive effects on soil fertility improvement and plant physiological characteristics. By compounding chicken manure with straw charcoal, we respectively set up six treatments: conventional fertilization (CK), conventional fertilization with straw charcoal (CF) and biochar-based fertilizers with straw charcoal ratios of 0%, 5%, 15%, and 25% (M 0C 0, M 1C 1, M 2C 2, M 3C 3). Through ecological stoichiometric analysis, we found that the biochar can increase soil C/N, C/P and N/P to varying degrees. In the 0-10cm and 10-20cm soil, C/P increased by 18.78% and 6.29%, respectively. Further homeostatic analysis found that C/P showed a strong homeostatic state between the surface and deep layers, indicating that the application of biochar could effectively enhance the fixation of phosphorus, and C/N and N/P showed a similar trend. Then, using Pearson correlation analysis and principal component analysis, a comprehensive evaluation of different biochar-based fertilizers was conducted. The results show that in the 0-10cm soil layer, the comprehensive scores of soil fertility are ranked as M 2C 2 > M 3C 3 > M 0C 0 > CF > M 1C 1 > CK, and in the 10-20cm soil layer, the scores are ranked as M 2C 2 > M 0C 0 > M 3C 3 > M 1C 1 > CK > CF. Therefore, biochar-based fertilizers made from poultry manure/straw charcoal have significant advantages in improving soil fertility.