Mammalian skeletal muscle is composed of different types of muscle fibers, which have different physiological and metabolic characteristics.The composition of muscle fiber types has a significant effect on the meat quality of livestock.In this study, we comprehensively analyzed the Whole transcriptome of the muscles Semitendinosus (glycolytic muscles、MS) and musculus longissimus (oxidative muscles、ML) of Lufeng cattle.Compared with glycolytic muscle, a total of 447 mRNAs, 87 lncRNAs and 18 circRNAs were differentially expressed in oxidative muscle.KEGG annotations showed that the differentially expressed mRNAs regulated by lncRNAs and circRNAs were mainly involved in PI3K-Akt, arachidonic acid metabolism, linoleic acid metabolism, calcium signaling pathway and thermogenesis signaling pathway, reflecting the difference between oxidative muscle and glycolytic muscle metabolites.In addition, regulatory networks related to muscle fiber type switching and mitochondrial energy metabolism were also constructed in the study.Our study provides new evidence for a better understanding of the molecular mechanisms underlying skeletal muscle fiber determination and meat quality characteristics in Lufeng cattle(LFC).