Figure 8 - Microscopic and IHC images of specimen of second patient (A) Microsection shows tumor cells arranged in cords, nests, trabeculae and rosette (arrows) pattern. The tumor is composed of round to elongated cells with moderate nuclear pleomorphism and brisk mitosis, [H&E, 40X]. (B) Stroma is predominantly sclerotic. Focal tumor cells show moderate to abundant clear cytoplasm, [H&E, 40X]. (C) On immunohistochemistry, Pan-CK is diffusely positive in the tumor cells. (D) Tumor cells show patchy positivity for EMA. (E) Diffuse membranous positivity is seen for CD99. (F) NKX2.2 is positive. (G) FLI-1 show strong nuclear positivity. (H) Tumor cells show patchy positivity for CK7.