6.2.8 Chlorination
Chlorination was carried out in one-litre amber glass bottles at room
temperature (21 ± 2 °C). Free chlorine was added to each litre of
simulated drinking water to achieve a Cl₂ concentration of 9.0 mg/L. UV
irradiation was conducted with a 254 nm wavelength light source from a
41 W low-pressure mercury lamp (Light Sources, Orange, CT, USA) (Huang
et al., 2019). A quasi-collimated beam with a 254 nm wavelength,
generated by 41 W low-pressure mercury lamps (Light Sources, Orange, CT,
USA), was used for UV irradiation (Huang et al., 2019). The comparison
of inactivation rates between antibiotic-resistant bacteria and general
heterotrophic bacteria during chlorination highlights the relative
resistance of antibiotic-resistant strains to chlorine. The bacterial
response, survival, and inactivation patterns of both heterotrophic and
antibiotic-resistant bacteria during chlorination were assessed. Data
suggest that ampicillin- and penicillin-resistant bacteria in secondary
wastewater are more susceptible to chlorine than other bacteria due to
bacterial inactivation (Templeton et al., 2009).