In this study, it was aimed to reveal the volume values of cervical segments in poultry. The total volume, substantia grisea and substantia alba volume values of each cervical segment of each animal were calculated using the Cavalieri’s Principle. Ten healthy adult female turkeys aged 8 months, and 10 healthy adult female guinea fowls, aged 8 months, were used. The animals were anesthetized and then perfused. Spinal cords were dissected. Length, weight and diameter measurements of each cervical segment were measured to obtain morphometric values. Each cervical segment was blocked with paraffin. Ten sections of 5 µm thickness were taken by a microtome, paralel and serial, with a step of 1/75 for each segment. The sections were stained with hematoxylin and eosin. The sections were photographed under a microscope with an objective x4. Shtereom I package program was used for volume calculations. This study revealed the presence of 13 cervical vertebrae and 14 cervical spinal segments in both turkey and guinea fowl. with this study, it was determined that the intumescentia cervicalis region in the turkey was between the eighth and the fourteenth cervical segments, and in the guinea fowl, between the nineteeth and the fourteenth cervical segments. With this study, the white matter, grey matter and total volume values of cervical spinal segments of turkey and guinea fowl were presented to the literature. Morphometric values of cervical segments were also calculated.