Wheat ( Triticum aestivum), a vital global staple and winter crop, significantly contributes to the GDP of agriculturally-based nations. However, it is severely impacted by Pyrenophora tritici-repentis ( Ptr), a fungus that produces Tox-A, a protein responsible for Tan spot disease. The RGD motif in Tox-A binds with high affinity to mesophyll cell receptors, triggering reactive oxygen species (ROS) production, leading to cell death and photosynthesis disruption. With the growing global population, addressing the threat posed by Ptr is crucial. In this study, we used computational approaches, including sequence retrieval, BLAST, and homology modeling via Swiss-Model, to explore Tox-A-like proteins (Tox-ALPs). Homology with Tox-A ranged from 96 - 100%, with ProSA Z-scores of -3.3 to -4.5. Phylogenetic analysis using MEGA 7 revealed that Tox-ALPs from three novel fungal species i.e ; Parastagonospora avenaria tritici, Bipolaris sorkiniana, and Parastagonospora nodorum are closely related to Ptr Tox-A and have the potential to induce similar Tan spot symptoms in wheat. It is elucidated that these species have potential agent for inducing Ptr Tox-A like symptoms in Wheat crop called Tan spot disease.