loading page

FpCBS is important for H2S production and plant infection in Fusarium pseudograminearum
  • +8
  • Jie Zhang,
  • Qiuyuan Chai,
  • Paul Goodwin,
  • Wenqian Zhu,
  • Mingcong Xia,
  • Runhong Sun,
  • Wen Xu,
  • Chao Wu,
  • Yajing Song,
  • Qianqian Dong,
  • Lirong Yang
Jie Zhang
Henan Academy of Agricultural Sciences
Author Profile
Qiuyuan Chai
Henan Academy of Agricultural Sciences
Author Profile
Paul Goodwin
University of Guelph School of Environmental Sciences
Author Profile
Wenqian Zhu
Henan Academy of Agricultural Sciences
Author Profile
Mingcong Xia
Henan Academy of Agricultural Sciences
Author Profile
Runhong Sun
Henan Academy of Agricultural Sciences
Author Profile
Wen Xu
Henan Academy of Agricultural Sciences
Author Profile
Chao Wu
Henan Academy of Agricultural Sciences
Author Profile
Yajing Song
Henan Academy of Agricultural Sciences
Author Profile
Qianqian Dong
Henan Academy of Agricultural Sciences
Author Profile
Lirong Yang
Henan Academy of Agricultural Sciences

Corresponding Author:luck_ylr@126.com

Author Profile

Abstract

Crown rot caused by Fusarium pseudograminearum remains a significant global challenge to wheat production. In this study, the role of FpCBS in F. pseudograminearum were investigated by creating deletion mutants and assessing their impacts on hydrogen sulfide (H 2S) production, oxidative stress adaptation, and pathogenicity, supported by transcriptomic and metabolomic analyses. Deletion of FpCBS resulted in a significant reduction in H 2S production, accompanied by increased levels of homocysteine and cysteine, suggesting the importance of FpCBS for H 2S biosynthesis. Furthermore, Δ Fpcbs mutants displayed diminished pathogenicity and decreased resistance to H 2O 2. Also, Δ Fpcbs mutants correlated with reduced catalase activity. Transcriptomic data revealed altered gene expression related to oxidative stress and virulence, including genes encoding cell wall-degrading enzymes, mycotoxins, and polyketides. Altogether, we proposed that F. pseudograminearum uses CBS-dependent H 2S production to facilitate host infection, adaptation to host-induced oxidative stress, and synthesis of virulence factors. These findings highlight the potential of FpCBS as a novel target for developing strategies to control wheat crown rot.
02 Sep 2024Submitted to Plant, Cell & Environment
16 Sep 2024Submission Checks Completed
16 Sep 2024Assigned to Editor
17 Sep 2024Review(s) Completed, Editorial Evaluation Pending
17 Sep 2024Reviewer(s) Assigned
08 Oct 2024Editorial Decision: Revise Minor