Gene expression profile and WGCNA
In this study, we investigated the collagen distribution in swim bladder
and other 8 tissues. There were 64 genes related to collagen that had a
TPM value greater than 10 in at least one sample, as shown inFig. 3a . The genes col1a1 , col1a2 , col6a6 and col10a1 were highly expressed in swim bladder. The WGCNA was
performed using 18,116 genes from 18 samples and 38 modules were
identified (Fig. 3b ). It was found that the four collagens were
all included in the blue module. Then the GO and KEGG analysis of blue
module was conducted, the results showed that top enriched GO terms,
including ion binding, extracellular matrix, growth and collagen trimer
(Fig. 3c ).