Transcriptome data Analysis
Transcriptome data were processed using Fastp (v0.23.2) for quality
control, Hisat2 (v2.1.0) for accurate transcript alignment, and
featureCounts (v2.0.6) for efficient quantification of transcript
abundance. These raw counts were subsequently converted into Transcripts
Per Million (TPM) values for normalization and comparative analysis. A
weighted gene coexpression network analysis (WGCNA) was performed using
the R (v4.3.3) package WGCNA (v1.72.5), following published procedure
(Langfelder & Horvath, 2008).