Genome assembly
By combining Hifi, Nanopore UL and Hi-C data, we assembled 686.86 Mb
genome sequences, consisting of 83 contigs with a contig N50 of 30.02
Mb. The GC content of genome is 41.34%, as same as the genome assembly
of C. lucidus, which has the GC content of 41.30% and 41.60%
(Table 2 ) (Gan et al., 2021). The length of contig N50 shows
the high quality assemble level of this genome, as it is much larger
than most of other Sciaenidae fish. For example, the length of N50 is
6.55 Mb in L. crocea, 10.7 Mb in Nibea coibor , and 26.58
Mb in C. lucidus (Gan et al., 2021; Mu et al., 2018; Yekefenhazi
et al., 2022). We use BUSCO to check the integrity of the assembled
genome. The result of BUSCO shows that we get 3,511 complete BUSOs,
which includes 3,478 complete and single-copy BUSCOs and 33 complete and
duplicated BUSCOs. There are also 52 fragmented BUSCOS and 77 missing
BUSCOs. There were no obvious false duplications (Table 2 ).