: A total of 17605 gene sequences were obtained from transcriptome sequencing, 12,368 predictable proteins, 278 transcription factors and 51 family information of transcription factors were obtained through database comparison; 10072 peptides were found in the process of proteome identification, and 1917 protein components were deduced from the peptides; we matched 6758 peptides to 33834 peptides in the transcriptome database in the process of peptide identification; we further detected the protein changes of whole scorpion tail and whole scorpion tail removal in the bulk and tonic by protein de novo sequencing. In the identification of peptidome, we found 6758 peptides in the transcriptome database, and 33834 peptides were identified by protein de novo sequencing; by further examining the protein changes in the tails of scorpion and the tail of scorpion in the powder and decoction, it was found that different parts of the drug and the method of drug administration may lead to changes in the function of scorpion, for example, if the scorpion is powdered into medicine in bulk, the protein components are preserved more intact, and the efficacy is closely related to inflammatory response and signal transduction; In the decoction, the protein components are destroyed and hydrolysed into amino acids and peptide segments, and the efficacy is closely related to the regulation of amino acids and the maintenance of energy and other function