Figure 5. Butyrate perturbs super-enhancers and preferentially suppresses SE-associated transcripts. (A) Super-enhancers (SE) were ranged by H3K27Ac signal in untreated human mast cells of donor 1 (left hockey stick graph) and donor 2 (right hockey stick graph). (B) Pathway enrichment analysis of 588 consistent (present in both donor 1 and 2) SE genes in untreated human mast cells. (C) Representative examples of SE (indicated by yellow rectangles) in untreated human mast cells (0 h, indicated in blue), that become hypo-acetylated (indicated by green bars) in response to butyrate treatment (24 h, indicated in red). Overlap between identified SE and hypo-acetylated regions is indicated by red bars (intersect). (D) Proportion of SE that contain hypo-acetylated regions (in blue) and SE regions that do not intersect with a hypo-acetylated region (in light red). (E) Pathway enrichment analysis of 502 hypo-acetylated SE genes. (F) The percentage of hypo-acetylated SE, typical enhancer (TE) or TSS regions linked to a downregulated gene. (G) Pathway enrichment analysis of 95 downregulated genes associated with hypo-acetylated SE regions. (H)