As the majority of hyper-acetylated peaks were located at typical enhancers (Fig. 3B-D), we next quantified changes in acetylation enrichment in the enhancer landscape (EL, defined as all non-TSS H3K27Ac peaks). Histone acetylation at the EL linked to the 864 downregulated and unchanged genes displayed an initial modest increase, but decreased again after 24 h (Fig. 4G, left and middle histogram). Acetylation at the EL around the 551 upregulated genes was increased at all measured timepoints (Fig. 4G, right histogram), which was supported by quantification of the area under the curve (Fig. 4H).