Figure 2. Butyrate triggers global histone hyper-acetylation without large-scale indirect effects on other histone modifications. Chromatin immunoprecipitation (ChIP)-Seq for histone 3 lysine 27 acetylation (H3K27Ac) and histone 3 lysine 4 di-methylation (H3K4Me2) was performed after 0, 3, 12 and 24 hours of butyrate treatment. (A) Megabase (Mb) coverage of histone acetylation (H3K27Ac, left) and methylation (H3K4Me2, right). Donor 1 (D1) is indicated in blue and donor 2 (D2) is indicated in red. Overlap between the donors is indicated by the shared peaks (yellow bars). (B) Total number of called acetylation (left) and methylation (right) peaks following butyrate treatment in donor 1 and donor 2, with shared peaks and increases in peak count (based on shared peak counts) indicated.