A 3 h exposure to butyrate led to 1226 significantly hypo-acetylated peaks, which were predominantly located near TSSs (Fig. 3B, within +/- 5kb). In fact, ~48% of all hypo-acetylated regions were located at a TSS (Fig. 3B, left donut graph). Although the total number of hypo-acetylated peaks strongly increased with prolonged butyrate exposure (8.8-fold increase), their location remained disproportionally enriched at TSS regions (Fig. 3B-D). Oppositely, butyrate treatment for 3 h led to 3547 significantly hyper-acetylated peaks, which were predominantly located distal of TSS regions (Fig. 3B, >5kb upstream or downstream). Overall, ~55% of all hyper-acetylated regions intersecting with a typical enhancer, and ~21% intersecting with a super-enhancer (Fig. 3B, right donut graph). Although the number of hyper-acetylated peaks increased with prolonged butyrate exposure (2.1-fold increase), their location remained disproportionally enriched at enhancer locations (Fig. 3B-D). Specifically, 12 and 24 h after butyrate treatment, ~86% of all hyper-acetylated peaks were located either at a typical enhancer or super-enhancer (Fig. 3C, D).