Supplementary Figure 5. H3K27Ac acetylation at super-enhancers and associated transcriptional dynamics. (A) The percentage of expressed hypo-acetylated genes that are downregulated upon butyrate treatment and associated with SE, TE or TSS. (B) Pathway enrichment analysis of 190 hypo-acetylated TE regions with repressed genes. (C) Proportion of identified SE that contain hyper-acetylated regions (in blue) and SE regions that do not intersect with a hyper-acetylated region (in light red). (D) The percentage of hyper-acetylated SE, TE or TSS regions linked to an upregulated gene. (E) Histograms of histone-acetylation at the TSS of upregulated genes with significant (left box) and non-significant (right box) hyper-acetylation at their TEs. The 0 h timepoint is indicated in light grey and TSS acetylation after butyrate treatment indicated in red. (F) Epigenetic explanation for butyrate-induced upregulation of gene expression. The proportion of upregulated genes with significant hyper-acetylated TSS (purple), TSS and SE (green), SE (orange), TE (yellow) and non-significant reduced acetylation (pink).