Figure 3. Butyrate induces distinct patterns of histone acetylation dynamics. (A) Distance of acetylated peaks in untreated human mast cells (0h) to associated transcription start site (TSS) regions, both upstream (left side) and downstream (right side). Distances of peak to TSS from 0 to 5 kb are indicated in purple, 5 – 50 kb in green and distances greater than 50 kb are indicated in yellow. Donut plots indicate the genomic locations of differentially acetylated regions, which were defined as typical enhancers (TE), super-enhancers (SE, identified using ROSE), TSS regions (TSS) or other. (B-D) Same analysis as in A but for mast cells treated with butyrate for 3 (B), 12 (C) and 24 (D) hours. Differential enrichment was calculated using DESeq2 on reproducible peaks (fold change > 2 and adjusted P value < 0.05).