Percentage of degranulation after IgE-mediated human mast cell activation in untreated, JQ-1 treated (50ng/mL, 24 h), butyrate treated (1mM, 24 h) or combination treated (JQ-1+butyrate, 24 h) cells. (C) Gene expression (2^delta CT) of LAIR, LCP2, LAT and LAT2 in human mast cells derived from two different donors treated with increasing concentration of JQ-1 for 24 h. Data represent mean ± SD (panel A), statistical significance was tested using a one-way ANOVA test: *Significantly decreased compared with control (P < .05). ***P < .001. NS, not significant.