Supplementary Figure 5. H3K27Ac acetylation at
super-enhancers and associated transcriptional dynamics. (A) The
percentage of expressed hypo-acetylated genes that are downregulated
upon butyrate treatment and associated with SE, TE or TSS. (B) Pathway
enrichment analysis of 190 hypo-acetylated TE regions with repressed
genes. (C) Proportion of identified SE that contain hyper-acetylated
regions (in blue) and SE regions that do not intersect with a
hyper-acetylated region (in light red). (D) The percentage of
hyper-acetylated SE, TE or TSS regions linked to an upregulated gene.
(E) Histograms of histone-acetylation at the TSS of upregulated genes
with significant (left box) and non-significant (right box)
hyper-acetylation at their TEs. The 0 h timepoint is indicated in light
grey and TSS acetylation after butyrate treatment indicated in
red. (F) Epigenetic explanation for
butyrate-induced upregulation of gene expression. The proportion of
upregulated genes with significant hyper-acetylated TSS (purple), TSS
and SE (green), SE (orange), TE (yellow) and non-significant reduced
acetylation (pink).