javascript:void(0)Butyrate preferentially suppresses super-enhancers and
their associated genes
Because super-enhancers can be extensively acetylated and
have been reported as preferential targets of HDAC
inhibitors 36,37, we next investigated
H3K27Ac dynamics at super-enhancers and transcriptional changes of their
target genes. From two biological replicates, 608 reproducible
super-enhancers were identified that were linked to 588 unique
associated genes in untreated primary human mast cells (Fig. 5A). These
genes were strongly enriched for immune effector cell processes, such as
cell activation, exocytosis and secretion (Fig. 5B). These included
super-enhancers associated with CD9, LAIR1, COTL1 and LAT, which are
known regulators of mast cell mediator secretion (Fig. 5C), as well as
other mast cell-associated genes such as KIT, MS4A2 and LYN
(Supplementary Table 2).