Figure 2. Butyrate triggers global histone
hyper-acetylation without large-scale indirect effects on other histone
modifications. Chromatin immunoprecipitation (ChIP)-Seq
for histone 3 lysine 27 acetylation (H3K27Ac) and histone 3 lysine 4
di-methylation (H3K4Me2) was performed after 0, 3, 12 and 24 hours of
butyrate treatment. (A) Megabase (Mb) coverage of histone acetylation
(H3K27Ac, left) and methylation (H3K4Me2, right). Donor 1 (D1) is
indicated in blue and donor 2 (D2) is indicated in red. Overlap between
the donors is indicated by the shared peaks (yellow bars). (B) Total
number of called acetylation (left) and methylation (right) peaks
following butyrate treatment in donor 1 and donor 2, with shared peaks
and increases in peak count (based on shared peak counts) indicated.