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Revisiting the Briggs ancient DNA damage model: a fast maximum
likelihood method to estimate postmortem damage
Abstract
Motivation: One essential initial step in the analysis of ancient DNA is
to authenticate that the DNA sequencing reads actually are from ancient
DNA. This is done by assessing if the reads exhibit typical
characteristics of postmortem damage, including cytosine deamination and
nicks. We present a novel statistical method implemented in a fast
multithreaded program, ngsBriggs, that enables rapid quantification of
PMD by estimation of the Briggs ancient damage model parameters (Briggs
parameters). Results: Using a multinomial model with maximum likelihood
fit, ngsBriggs accurately estimates the parameters of the Briggs model,
quantifying the PMD signal from single and double-stranded DNA
regions.We extend the original Briggs model to capture PMD signals for
contemporary sequencing platforms and show that ngs- Briggs accurately
estimates the Briggs parameters across a variety of contamination
levels. Classification of reads into ancient or modern reads, for the
purpose of decontamination, is significantly more accurate using
ngsBriggs than using other methods available. Furthermore, ngsBriggs is
substantially faster than other state-of-the-art methods. ngsBriggs
offers a practical and accurate method for researchers seeking to
authenticate ancient DNA and improve the quality of their data.