Endocrine disrupting chemicals (EDCs) are chemicals that interfere with the normal functioning of the endocrine (hormone) system in humans or other organisms. EDCs can exist in the environment in various ways. Exposure of organisms (including humans) to EDCs in the environment has been associated with various health problems, including feminization of fish, reproductive problems in alligators, decreased sperm count in humans, and childhood obesity. The aim of this study is to develop an environmentally friendly liquid-liquid extraction method for the determination of bisphenol A (BPA) and estradiol (E2) due to their frequency of occurrence, environmental importance, and chemical similarity to other EDCs, and to determine them using the LC-MS/MS method. A green LC-MS/MS method has been developed for the simultaneous determination of BPA and estradiol, which are important EDCs. The solvents used in the liquid-liquid extraction method developed and optimized in this study have environmentally friendly, easy degradability, and low toxicity properties. Ethyl acetate-acetone (4/1, v/v) was selected as the extraction solvent pair in the optimized extraction method. The best responses were obtained with a 10% NaCl concentration and 10 seconds of vortex time. The linear range of the developed method was found to be between 12 pg/mL and 100 ng/mL for BPA and between 48 pg/mL and 100 ng/mL for estradiol (E2). The intra-day and inter-day repeatability of the method is within an acceptable range at the internal level. The percent recovery values for both analytes were found to be between 75-98%. BPA and E-2 were successfully determined in drinking and environmental waters using the developed method.