Detergent-mediated viral inactivation is an important process step for ensuring viral safety of parenteral biotherapeutics, including plasma proteins and monoclonal antibodies (mAb). The conventional Triton™ X-100 detergent has ecological toxicity concerns and REACH classification that mandate replacement in the biopharmaceutical industry. Criteria for a replacement detergent include viral inactivation efficacy, acceptable safety and biodegradation profile, process removal and quality suitable for parenteral drug product manufacturing. A non-ionic, C11-15 secondary alcohol ethoxylate, Deviron ^® 13-S9 detergent, has been demonstrated to meet the necessary requirements for detergent performance. Benchmarking studies with Triton™ X-100 demonstrate comparable performance with a panel of enveloped viruses in multiple matrices, including human IgG, clarified cell culture harvest, and fractionated plasma. Deviron ^® 13-S9 detergent demonstrated viral inactivation efficiency comparable to or better than, Triton™ X-100 detergent, achieving > 5 log reduction values (LRV). Critical micelle concentration (CMC) was determined across different temperatures and media. Deviron ^® 13-S9 detergent was demonstrated to be readily biodegradable according to OECD 301B guidelines. Effective removal with typical chromatography processes used in downstream purification was confirmed. These findings support Deviron ^® 13-S9 detergent as a viable alternative to Triton™ X-100 detergent, ensuring robust viral inactivation, environmental compatibility, and alignment with regulatory requirements.