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In vivo CRISPR-Cas9 expression in Candida glabrata , Candida bracarensis and Candida nivariensis : a versatile tool to study chromosomal break repair.
  • Killian Métivier,
  • Youfang Zhou-Li,
  • Cécile Fairhead
Killian Métivier
IFP Energies nouvelles
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Youfang Zhou-Li
IDEEV
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Cécile Fairhead
IDEEV

Corresponding Author:cecile.fairhead@universite-paris-saclay.fr

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Abstract

The CRISPR-Cas9 system is extremely useful for genome editing in many species, including the model yeast Saccharomyces cerevisiae, and other yeast species. We have previously reported the use of an inducible CRISPR-Cas9 system in Candida glabrata, which allows genome editing but also the study of Double Strand Break (DSB) repair. We report, in this study, a comparable system for C. glabrata, relying on a new plasmid, which is more stable than the previous one. We also report the use of this plasmid to induce DSBs in two additional human pathogens, Candida bracarensis and Candida nivariensis. We examine lethality induced by an in vivo DSB in the three species and describe the different types of Non-Homologous End-Joining (NHEJ) events detected in these three pathogens.
Submitted to Yeast
14 Jun 2024Reviewer(s) Assigned
08 Jul 2024Review(s) Completed, Editorial Evaluation Pending
10 Jul 2024Editorial Decision: Revise Minor
17 Jul 20241st Revision Received
18 Jul 2024Submission Checks Completed
18 Jul 2024Assigned to Editor
18 Jul 2024Review(s) Completed, Editorial Evaluation Pending
27 Jul 2024Editorial Decision: Accept