Animals
All procedures complied with European Rules and Guidelines
(2012/707/EU). The work in Lisbon also complied with the Portuguese
legislative action (DL 113/2013) for the protection of animals used for
scientific purposes, being authorized by the Animal Welfare Body of the
Instituto de Medicina Molecular João Lobo Antunes (ORBEA-iMM). The
animals (Wistar rats, Sprague-Dawley rats and C57BL/6J strain mice, as
specified below) were housed in a strictly controlled iMM rodent
facility, as authorized by the Portuguese authority for Animal Welfare
(Direção-Geral de Alimentação e Veterinária - DGAV). The work
using female Xenopus laevis frogs to obtain oocytes (Sapienza
University, Rome, Italy) conformed to institutional policies and
guidelines of the Italian Ministry of Health (no. authorization
427/2020-PR).