Author Contribution: AMS conceived the study; KAJ, EP, GR, MJD
and JAR contributed to the discussion of the ongoing experiments; DKT
synthesized MRS5474; AG and AMS performed the fEPSP recording
experiments and the GABA uptake studies; GABA uptake studies also
received input from NR, TPM and SHV; LR-R prepared the organotypic
slices cultures, being trained in this by CAV, and performed the field
potential recordings of organotypic slices; LR-R also performed Western
Blot and induced the in vivo model of epilepsy, being trained in
this by SX. LR-R and BB created the program to analyze the field
potential recordings of organotypic slices. GR, AG and VA performed the
experiments involving recordings of GABAergic currents from oocytes
injected with human tissue samples; CD and DMR performed the EPSC
recording experiments; AR-C, CB and EA were involved in the selection of
human tissue samples making them available for the study. AMS, KAJ, EP,
AG, LR-R, GR and CD contributed to MS writing, conceived Tables and
Figures. All authors read the final version of the MS contributing to
its final form.
Acknowledgements :
The collaborative work in Lisbon, Rome and Amsterdam was supported by
H2020-WIDESPREAD-05- 2017-Twinning (EpiEpinet) under grant agreement No.
952455, and the NIDDK Intramural Res. Program ZIADK031117. The work in
Lisbon was supported by PTDC/MED-FAR/30933/2017 and
PTDC/MED-FAR/4834/2021 from Fundação para a Ciência e a Tecnologia (FCT)
and by the International Society for Neurochemistry (Carer Development
Grant 2021 to SHV). AG received an iMM scholarship (iMM-BI-4-2022).
L.R-R. received a fellowship from FCT (PD/BD/150344/2019). The work in
Rome was supported by the Fondi Ateneo grants, nos. RM11916B84D24429
(EP), RG12117A8697DCF1 (EP, GR) funded by Sapienza University, PRIN 2022
(EP, GR) and PRIN-PNRR 2022 (EP) from the Italian Ministry of University
and Research. GR and VA were also supported by funding of the Italian
Ministry of Health, “Ricerca corrente”. This study has not been
preregistered.