No Drug: refers to data from slices not treated with any drug. MRS5474:
refers to data from slices in the presence of MRS5474 (250nM) for 30
minutes before first analysis time (50–60 minutes). In all cases and
for all parameters, the analysis at time 10–20 (baseline, pre-drug
condition) in each slice was normalised to 1. Statistically significant
effects of MRS5474 when compared with pre-drug condition in the same
slices are highlighted in red; *P<0.05, **P<0.01
(one-way ANOVA followed by Dunnett’s multiple comparisons test). When
both time and drug condition were simultaneously analyzed as independent
variables, an effect of MRS5474 upon number of bursts also emerged
(highlighted in blue, *P<0.05, comparison between the same time
frame within slices, in the absence or presence of the drug; two-way
ANOVA followed by Sidak’s multiple comparison test), while the effect of
MRS5474 vs baseline in the frequency of bursts still hold significance.
Note that data for the analysis of the amplitude and frequency of spikes
within the burst did not include values with zero bursts (i.e. fully
blocked by MRS5474), since the frequency and the amplitude of spikes
were not independent from the burst occurrence itself. Data from those
experiments are included in Figure 3, where individual data points are
represented.
Table 2. Clinical information of tissue samples from patients who
underwent hippocampal focal resection (EHS1-8) or from autopsy (CHS1-6),
used for Western Blotting.