Author Contribution: AMS conceived the study; KAJ, EP, GR, MJD and JAR contributed to the discussion of the ongoing experiments; DKT synthesized MRS5474; AG and AMS performed the fEPSP recording experiments and the GABA uptake studies; GABA uptake studies also received input from NR, TPM and SHV; LR-R prepared the organotypic slices cultures, being trained in this by CAV, and performed the field potential recordings of organotypic slices; LR-R also performed Western Blot and induced the in vivo model of epilepsy, being trained in this by SX. LR-R and BB created the program to analyze the field potential recordings of organotypic slices. GR, AG and VA performed the experiments involving recordings of GABAergic currents from oocytes injected with human tissue samples; CD and DMR performed the EPSC recording experiments; AR-C, CB and EA were involved in the selection of human tissue samples making them available for the study. AMS, KAJ, EP, AG, LR-R, GR and CD contributed to MS writing, conceived Tables and Figures. All authors read the final version of the MS contributing to its final form.
Acknowledgements :
The collaborative work in Lisbon, Rome and Amsterdam was supported by H2020-WIDESPREAD-05- 2017-Twinning (EpiEpinet) under grant agreement No. 952455, and the NIDDK Intramural Res. Program ZIADK031117. The work in Lisbon was supported by PTDC/MED-FAR/30933/2017 and PTDC/MED-FAR/4834/2021 from Fundação para a Ciência e a Tecnologia (FCT) and by the International Society for Neurochemistry (Carer Development Grant 2021 to SHV). AG received an iMM scholarship (iMM-BI-4-2022). L.R-R. received a fellowship from FCT (PD/BD/150344/2019). The work in Rome was supported by the Fondi Ateneo grants, nos. RM11916B84D24429 (EP), RG12117A8697DCF1 (EP, GR) funded by Sapienza University, PRIN 2022 (EP, GR) and PRIN-PNRR 2022 (EP) from the Italian Ministry of University and Research. GR and VA were also supported by funding of the Italian Ministry of Health, “Ricerca corrente”. This study has not been preregistered.