Animals
All procedures complied with European Rules and Guidelines (2012/707/EU). The work in Lisbon also complied with the Portuguese legislative action (DL 113/2013) for the protection of animals used for scientific purposes, being authorized by the Animal Welfare Body of the Instituto de Medicina Molecular João Lobo Antunes (ORBEA-iMM). The animals (Wistar rats, Sprague-Dawley rats and C57BL/6J strain mice, as specified below) were housed in a strictly controlled iMM rodent facility, as authorized by the Portuguese authority for Animal Welfare (Direção-Geral de Alimentação e Veterinária - DGAV). The work using female Xenopus laevis frogs to obtain oocytes (Sapienza University, Rome, Italy) conformed to institutional policies and guidelines of the Italian Ministry of Health (no. authorization 427/2020-PR).