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Biological and Biophysical Methods for Evaluation of Inhibitors of sortase A in Staphylococcus aureus : An Overview
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  • Debajit Dewan,
  • Aaheli Basu,
  • Debraj Dolai,
  • SARMISTHA PAL
Debajit Dewan
JIS University
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Aaheli Basu
JIS University
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Debraj Dolai
JIS University
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SARMISTHA PAL
JIS University

Corresponding Author:sarmisthapal1991@gmail.com

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Abstract

Staphylococcus aureus, one of the most notorious pathogens, develops antibiotic resistance by formation of a thick layer of exopolysaccharides known as biofilms. Sortase A, a transpeptidase responsible for biofilm formation and attachment to the host surface, has emerged as an important drug target for development of anti-virulence agent. A number of sortase A inhibitors, both peptide and non-peptides are reported which involved the use of several experiments which may provide insights regarding binding affinity, specificity, safety and efficacy of ligands. In this review, we focus on the principles, pros and cons, and the type of information obtained from biophysical (FRET assay, Microscale Thermophoresis, Surface Plasmon resonance, CD spectroscopy etc.) and biological (Cell viability assay, biofilm formation assay, CLSM, Western blot analysis, in vivo characterization on mice etc.) methods for estimation of probable sortase A inhibitors, which might be helpful to the researchers who might be interested to delve into the development of sortase A inhibitors as a drug, to address the burning question of Anti-microbial Resistance (AMR).
07 Jun 2024Submitted to Cell Biochemistry & Function
17 Jun 2024Submission Checks Completed
17 Jun 2024Assigned to Editor
08 Aug 2024Review(s) Completed, Editorial Evaluation Pending
16 Aug 2024Editorial Decision: Revise Major
02 Sep 20241st Revision Received
02 Sep 2024Review(s) Completed, Editorial Evaluation Pending
02 Sep 2024Submission Checks Completed
02 Sep 2024Assigned to Editor
08 Sep 2024Reviewer(s) Assigned
02 Oct 2024Editorial Decision: Accept