Aggregation-based Dual-Target Probe for Dual-Color Super-Resolution
Monitoring Mitophagy and Evaluating Drugs Regulating Mitochondria
Abstract
Developing single fluorescent probe for simultaneously visualizing
mitophagy flux and oxidative stress with super-resolution is highly
demanded yet quite challenging. Herein, we design and synthesize a
ratiometric HClO probe AHOH, which is capable of simultaneously
staining lysosomes and mitochondria with red and green color,
respectively. AHOH could be selectivity oxidized by HClO,
leading to a large emission blue shift (90 nm) and an over 1300-fold
enhancement of the emission ratio of Fl547
nm/Fl637 nm. We apply AHOH in
super-resolution microscopy and clearly visualize the dynamics of
mitochondria-lysosomes interactions and the oxidative stress states upon
different stimuli. Mitochondria dysfunction triggered by different drugs
and genetic defect lead to elevated oxidative stress and higher levels
of mitophagy. Moreover, AHOH could serve as a reliable tool for
evaluating the efficacy of drugs regulating mitochondria dysfunction.
This work provides a powerful dual-color super-resolution imaging agent
for real time monitoring the dynamics of organelle interactions and
oxidative stress.