Purpose: Due to its increased volume, polycystic ovarian tissue is more prone to torsion than normal ovarian tissue. In treating ovarian torsion, detorsion is applied to ensure oxygenation of hypoxic tissues. However, the resulting oxygen radicals cause tissue damage. Bromelain is a substance obtained from pineapple, and studies in the literature show it is used as an antioxidant. This study aimed to evaluate the damage caused by ischemia-reperfusion (I/R) in the torsion-detorsion model created in normal and polycystic ovarian tissue and investigate the role of bromelain in this damage.Methods: Polycystic ovarian tissue was created by applying dihydroepiandrosterostenodione sulfate to a group of rats. Afterward, a torsion-detorsion model was created in all rats. Bromelain was administered to a group of rats, and its effect on I/R injury was evaluated. After the procedure, tissues were taken, histopathological examination was performed, and MDA, TUNEL, and NF-κB levels were measured.Results: This study detected significant decreases in MDA and NF-κB levels and the percentage of apoptotic cells evaluated by TUNEL staining in rats with IR damage and given bromelain, compared to the control group. It has also been shown that I/R damage is more pronounced in polycystic ovarian tissue than in normal ovarian tissue.Conclusion: Ischemia perfusion damage may be more pronounced in polycystic ovarian tissue than in normal ovarian tissue. Bromelain may be preferred to prevent I/R injury caused by ovarian torsion. It is also thought that bromelain may function in treating polycystic ovaries, and further studies can be conducted on this subject.Keywords: Bromelain, ischemia‒reperfusion, ovarian torsion, polycystic ovary.