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Adipogenic differentiation effect of human periodontal ligament stem cell initial cell density on autologous cells and human bone marrow stromal cells
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  • Yuxing Bai,
  • Jing Wang,
  • Yingzhi Gu,
  • Yaxi Sun,
  • Qingchen Qiao,
  • Xiaofeng Huang,
  • Kai Yang
Yuxing Bai
Beijing Stomatological Hospital

Corresponding Author:byuxing@ccmu.edu.cn

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Jing Wang
Beijing Stomatological Hospital
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Yingzhi Gu
Beijing Stomatological Hospital
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Yaxi Sun
Beijing Stomatological Hospital
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Qingchen Qiao
Beijing Stomatological Hospital
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Xiaofeng Huang
Capital Medical University Affiliated Beijing Friendship Hospital
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Kai Yang
Beijing Stomatological Hospital
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Abstract

Stem cells have differentiation and regulatory functions. Here, we discuss the effect of cell culture density on stem cell proliferate, adipogenesis and regulation ability. To investigate the effect of the initial culture density of human periodontal ligament stem cells (hPDLSC) on the adipogenic differentiation of autologous cells. We found that the proliferation rate of hPDLSC increased with the initial cell densities (0.5~8 × 10 4 cells/cm 2) increase. After adipogenic differentiation induced by different initial cell densities of hPDLSC, we found that the mean adipose concentration and the expression levels of lipoprotein lipase (LPL), CCAAT/enhancer binding protein α (CEBP-α) and peroxisome proliferator-activated receptor γ (PPAR-γ) genes all increased with increasing cell density. To investigate the regulatory role of hPDLSCs on the adipogenic differentiation of other cells, we used secreted exosomes derived from hPDLSCs cultured at different initial cell densities of 50 μg/mL to induce the adipogenic differentiation of human bone marrow stromal cells (hBMSC). We also found that the mean adipose concentration and the expression of LPL, CEBP-α and PPARγ genes increased with increasing cell density, the optimal culture density was 8 × 10 4 cells/cm 2. This provides a laboratory research basis for the application of adipogenic differentiation of stem cells.
11 Apr 20241st Revision Received
11 Apr 2024Submission Checks Completed
11 Apr 2024Assigned to Editor
11 Apr 2024Review(s) Completed, Editorial Evaluation Pending
15 Apr 2024Reviewer(s) Assigned
09 May 20242nd Revision Received