Species delimitation
The genetic distances among all COI sequences varied from 0 to 2% (Supplementary Material). The mean intraspecific genetic distance amongA. alalia was 0.04%, and for A. mantiqueira, it was 0.29%. No “barcode gap” was found between inter- and intraspecific genetic distances since the estimated mean interspecific distance was 0.17%. Comparatively, the genetic distances among all SNPs sequences varied from 3.16% to 35%. Regarding the genetic distances estimated with SNPs, there is a substantial difference between intraspecific (A. alalia = 10.72%; A. mantiqueira = 11.39%) and interspecific mean distances (31.52%). The ABDG method applied to thebarcode sequences recovered only one group that comprises all individuals sampled for the two species. However, the PTP and bPTP methods (Fig. 6) recovered 16 groups, a large one including individuals of both species and 15 groups with one individual each.