Barcode analyses
The TCS haplotype network (Fig. 5) resulted in six haplotypes: the most common haplotype is shared between most individuals of the two species, three of them are exclusive for A. mantiqueira , and two of them are exclusive for A. alalia . The highest number of mutational steps found between two haplotypes was six. The Bayesian inference (Fig. 5) based on COI sequences failed to recover clades for the two species or sampling localities.