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Integrated metabolomic, lipidomic and proteomic analysis define the metabolic changes occurring in curled areas in leaves with leaf peach curl disease
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  • María Angelina Novello,
  • Claudia Bustamante,
  • Laura A. Svetaz,
  • Camila Goldy,
  • Gabriel Valentini,
  • Maria Drincovich,
  • Yariv Brotman,
  • Alisdair R. Fernie,
  • María Lara
María Angelina Novello
Centro de Estudios Fotosinteticos y Bioquimicos
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Claudia Bustamante
Centro de Estudios Fotosinteticos y Bioquimicos
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Laura A. Svetaz
Centro de Estudios Fotosinteticos y Bioquimicos
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Camila Goldy
Centro de Estudios Fotosinteticos y Bioquimicos
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Gabriel Valentini
Instituto Nacional de Tecnologia Agropecuaria
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Maria Drincovich
Centro de Estudios Fotosinteticos y Bioquimicos
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Yariv Brotman
Tel Aviv University School of Plant Sciences and Food Security
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Alisdair R. Fernie
Max-Planck-Institut fur Molekulare Pflanzenphysiologie
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María Lara
Centro de Estudios Fotosinteticos y Bioquimicos

Corresponding Author:lara@cefobi-conicet.gov.ar

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Abstract

Peach Leaf Curl Disease, caused by the fungus Taphrina deformans, is characterized by reddish hypertrophic and hyperplasic leaf areas. To comprehend the biochemical imbalances caused by the disease an integrated approach including metabolomics, lipidomics, proteomics and complementary biochemical techniques was undertaken. Symptomatic and asymptomatic areas were dissected from leaves with increasing extension of the disease. A differential metabolic behaviour was identified in symptomatic areas with respect to either asymptomatic areas or healthy leaves. Symptomatic areas showed an altered chloroplastic functioning and composition which differs from the typical senescence process and includes decrease in the photosynthetic machinery, alteration in plastidic lipids, and decreased starch, carotenoid and chlorophyll biosynthesis. In symptomatic areas, alteration in redox-homeostasis proteins and in triacylglycerols content, peroxidation and double bond index were observed. Proteomic data revealed induction of host enzymes involved in auxin and jasmonate biosynthesis together with up-regulation of phenylpropanoid and mevalonate pathways and down-regulation of the plastidic methylerythritol phosphate route. Amino acid pools were affected, with up-regulation of proteins involved in asparagine synthesis. Overall, we conclude that curled areas exhibited a metabolic shift towards functioning as a sink tissue importing sugars and producing energy through fermentation and respiration and reductive power via the pentose phosphate route.
Submitted to Plant, Cell & Environment
Submission Checks Completed
Assigned to Editor
Reviewer(s) Assigned
21 Jun 2024Review(s) Completed, Editorial Evaluation Pending
06 Jul 2024Reviewer(s) Assigned
22 Jul 2024Editorial Decision: Revise Minor
27 Aug 20241st Revision Received
28 Aug 2024Submission Checks Completed
28 Aug 2024Assigned to Editor
28 Aug 2024Review(s) Completed, Editorial Evaluation Pending
08 Sep 2024Reviewer(s) Assigned
28 Sep 2024Editorial Decision: Accept