Conclusion
This study demonstrated that Anopheles mosquito species
recognition may be accomplished with success using molecular
characterization. DNA barcoding results, which utilized the genetic
marker 18S rDNA, were equivalent to the morphological identification of
some species and also allowed for identification of morphologically
misidentified species. It is crucial to highlight that in situations
when conventional morphological classification was unsuccessful owing to
damaged specimens and unidentifiable characteristics, DNA barcoding
successfully identified these species. The newly discovered
sequence could potentially be utilized as reference sequences in future
mosquito taxonomy investigations. The successful identification of
numerous mosquito species in this study will assist with malaria
research by determining the real and prospective danger of arbovirus or
parasite transmission, as well as providing fundamental knowledge for
vector surveillance and enhancing vector control strategies. In the
future, molecular identification implementations should include
barcoding a greater number of species and incorporating other genetic
markers that improve the discriminatory strength of this identification
approach. DNA barcoding might also be used in conjunction with next
generation sequencing to identify huge numbers of mosquitos at
once, considerably reducing the processing time required for species
characterization.