3.4. Analysis of Cell-Based Reporter Gene Bioassay
We assessed the effect of forced degradation methods on the products, which was detected using a cell-based reporter gene assay (RGA). The change of Raman spectra on the products showed similar results to those shown by RGA. Different processing methods of the products were tested in the bioassay, and the IC50 was calculated between the treated and untreated products.We used different concentrations of samples (50%, 75%, 90%, and 95%) to evaluate the biological activity as system adaptability conditions(Figure 3a,b). These results are within the range of the theoretical results.The method of RGA can use evaluate samples of forced degradation. 12-and 24-h light treatments did not significant difference with reference(Figure 3b). 48-and 96-h light treatments,12-h heat treatments were little difference with reference(Figure 3c). 24-,48- and 96-h heat treatments were significant difference with reference(Figure 3d).