Figure 3. Validation of the RGA bioassay. (a ) Four different sample concentrations were seeded to be inhibited by the VEGF (primary concentration: 100, 95, 90, and 75 µg/mL; dilution ratio: 1:4). The inhibition curves of the sample are shown. (b ) The primary concentration for 12-h light treatment, 24-h light treatment, and positive control was 100 µg/mL; the primary concentration of the 50% sample was 50 µg/mL; dilutions of 1:4 were used to construct four inhibition curves; (c ) The primary concentration for 48-h light treatment, 96-h light treatment, 12-h heat treatment, and positive control was 100 µg/mL; dilutions of 1:4 were used to construct four inhibition curves; (d ) The primary concentration for 24-h heat treatment, 48-h heat treatment, 96-h heat treatment, and positive control was 100 µg/mL; dilutions of 1:4 were used to construct four inhibition curves. RGA, reporter gene assay; VEGF, anti-vascular endothelial growth factor.
The bioassay results for the bioactivity were successfully evaluated by RGA.The results of the bioactivity are shown in Table 4.
Table 4. The result of the bioacitivity.