3.4. Analysis of Cell-Based Reporter Gene Bioassay
We assessed the effect of forced degradation methods on the products,
which was detected using a cell-based
reporter gene assay (RGA). The
change of Raman spectra on the products showed similar results to those
shown by RGA. Different processing methods of the products were tested
in the bioassay, and the IC50 was calculated between the treated and
untreated products.We used different concentrations of samples (50%,
75%, 90%, and 95%) to evaluate the biological activity as system
adaptability conditions(Figure 3a,b). These results are within the range
of the theoretical results.The method of RGA can use evaluate samples of
forced degradation. 12-and 24-h light treatments did not significant
difference with reference(Figure 3b). 48-and 96-h light treatments,12-h
heat treatments were little difference with reference(Figure 3c).
24-,48- and 96-h heat treatments were significant difference with
reference(Figure 3d).