3.3. Analysis of Charge Heterogeneity
To further evaluate the products of forced degradation, we used cation exchange high performance liquid chromatography as important indicators to verify the product of forced degradation, which were used to assess the specificity of activity identification by Raman.
The results are shown in Table 3. According to the profiles, the acid, main, and basic peaks showed significant changes (Figure 2).The acid isomers were increased to 57.89%,The basic isomers were increased to 5.66% and The main charge isomer were decreases to 36.45%,which will affect the biological activity and effectiveness of the product.
Table 3. The result of the Charge Heterogeneity of samples