Rationale: Laser ablation isotope ratio mass spectrometry is a technique that is increasingly being applied for high-resolution analysis of carbon stable isotopes in organic materials. Here we present a new LA-IRMS facility housed in the Research School of Earth Sciences, Australian National University, Canberra, following the development and implementation of similar facilities elsewhere. The LA-IRMS technique allows for the measurement of stable isotopes within incredibly challenging tree ring chronologies, of which rings may be only several cells wide. Methods: We tested two commonly used standard materials, including IAEA-C3 holocellulose and VWR gel blotting pads, in the new LA-IRMS system. Additionally, we analysed a 13-year tree ring sample of Eucalyptus pauciflora subsp . niphophila to test the intra-annual capability of the LA-IRMS. Results: IAEA-C3 holocellulose ( n = 35) and VWR gel blotting pads ( n = 27) produced δ 13C ‰ (VPDB) values of -24.73 ± 0.08 ‰ and -26.56 ± 0.13 ‰, which agreed with published values (IAEA-C3: -24.72 ‰, VWR: -26.57 ‰). The LA-IRMS system produced a single-tree δ 13C ring series with high intra- and inter-annual variability. Conclusions: Our results demonstrate the potential for ultra-high temporal resolution analysis of stable-isotope dendrochronological samples using the new LA-IRMS. This facility offers an avenue for the expansion of tree ring research in Australia, supporting a field that continues to face challenges associated with poor ring definition in much of the endemic flora. In addition to this, the LA-IRMS facility has the potential to expand into the measurement of other organic and inorganic for their stable isotopic signal, for example the calcareous material of molluscs.