The self-silencing theory explains how cultural norms expect women to maintain their relationships by silencing their self, which can restrict voice and power in relationships, thereby leading to depression. However, the literature regarding gender differences in self-silencing is inconsistent. In this study we aimed to investigate gender and cultural differences in self-silencing in an adult community sample. Iranians residing in Australia (men n = 60, women n = 58) and European Australians (men n = 53, women n = 59) completed measures of self-silencing, depression and self-reported gender. There were no cultural or gender differences in self-silencing. Aspects of self-silencing (i.e., divided self and externalized self-perception) were positively associated with depressive symptoms. Gender moderated the association between care as a self-sacrifice and depressive symptoms, with the association being negative for men but positive and significant for women. Cultural group was not found to moderate any of the associations. This cross-cultural study offers further insights regarding self-silencing in the context of depression and provides initial insights for clinical practice. Keywords: Self-silencing, gender, depression, culture