Atmospheric and room temperature plasma (ARTP) mutagenesis technology have a rapid development in recent years due to advantages of simple operation, safety, environmental-friendly, high mutation rate, large-capacity mutation library, etc. It has been applied in the fields of food, agriculture and medicine. Data of 167 articles, related to ARTP mutagenesis technology from 2011 to 2021, were collected from the Web of Science Core Collection (WOSCC), visually analyzed the keyword and reference of articles by CiteSpace. Several research hotspots of ARTP mutagenesis technology were found.