Anxiety can also be termed as excessive worry which last for at least more than six months and patients can have difficulty in controlling them. Anxiety is having 31% of life time prevalence and sad part is that the anxiety disorders are not treated and diagnosed well. 3.1% which is 6.8 Million peoples of America are having anxiety disorders. The main part of clove oil is eugenol along with caryophyllene. Eugenol is present at about 70 to 90 percentage and also it contains 15 percentage of total dry weight of bud. METHODOLOGY: Pre-clinical experimental study was conducted for 10-12 Months. Male BALB/c mice were purchased from ICCBS. Sample Size was 42 BALB/c mice, divided into 07 groups. Elevated plus maze and open field tests were used to asses behavioral activities and results was measured as Mean and SD, frequency and percentages, conclusion: The animal model was developed to assess whether Eugenol and its liposomal based nano carrier protect anxiety disorders in mice or vice versa. Therefore with the help of behavioral studies i.e. open field test and elevated plus maze test, eugenol and its liposomal based nano particle protect anxiety disorder in the mice by increasing the entry time and stay of mice in open arms for longer duration in elevated maze plus test and in open field the anxiety was reduced after mice spent most of time in the center rather than periphery.