Background: The information received by patients with cancer and the consequent satisfaction are main components of the evaluation of quality of care. We aim to validate the Mexican-Spanish version of the QLQ-INFO25 in patients with gastrointestinal cancer. Methods: Patients included responded the QLQ-INFO25 instrument at a cancer center. The usual psychometric tests were performed. Results: 70 female and 66 males were included (mean age 56.2); 111 were colorectal and 25 gastroesophageal cancer. Questionnaire compliance rates were high and the instrument was well accepted. No missing values were found. Convergent item correlation was 0.008 to 0.768 and divergent item correlation was from 0.014 to 0.662. Cronbach’s α coefficients of multi-item scales were >0.7 (range, 0.655–0.905). Four scales distinguished among esophagogastric or colorectal cancer, and four scales were associated with overall survival. Conclusion: The Mexican-Spanish version of the questionnaire is reliable and valid to be used in clinical trials.