Objective, To evaluate clinical features of children with Deep venous thrombosis (DVT) associated with acute hematogenous osteomyelitis (AHO) caused by Staphylococcus aureus. Method, We reviewed a 4-year medical records of patients with DVT of AHO conclusive caused by Staphylococcus aureus (S. aureus) retrospectively and we compared clinical and laboratory characteristics of AHO with or without DVT, and patients whose DVT dissolved within or more than 3 weeks. Results, 87 AHO patients were admitted during four years period. Nineteen of 87 (22%) patients had DVT. For DVT patients, median age was 9 years (range, 6 mouths-15 years). 74% (14/19) patients were boys. Methicillin-susceptible Staphylococcus aureus (MSSA) accounted for 58% (11/19). All DVT occurred adjacent to the infection site. Femoral vein and common femoral vein were both the two most affected vein (9 cases, respectively). Eighteen (95%) patients received anticoagulation therapy with low molecular weight heparin. Seven of the 13 (54%) with available data had complete resolution of DVT within three weeks of anticoagulation therapy. None of patients required rehospitalization for bleeding or recurrent of DVT. Comparative analysis found patients with DVT were older. C-reactive protein, procalcitonin, D-dimer, fibrinogen, positive blood culture and incidence of intensive care unit admission, multifocal, the length of hospital stay were higher in patients with DVT. Conclusion, More than twenty percent of patients with S. aureus AHO developed DVT in present study. MSSA accounted more than half. More than half cases had complete resolution of DVT within three weeks of anticoagulation therapy without complications afterwards.