Background: Endotracheal intubation is a procedure commonly performed in the emergency department. Endotracheal intubation poses a risk of exposure to infectious aerosol droplets. The present study aims to test the effect of using an aerosol box and personal protective equipment on the intubation time while performing on endotracheal intubation manikin. Methods: The study participants (11 emergency specialist, 11 emergency physician and 11 general practitioner) performed endotracheal intubation on a training manikin in three different airway simulations. Simulation 1 had neither PPE nor AB, simulation 2 had PPE, and simulation 3 had both PPE and AB. The intubation times, the number of intubation attempts, and the discomfort caused by the aerosol box were recorded. Results: There was no significant difference in the number of intubation attempts between the physicians according to their position and airway simulations (p>0.05). There was a significant difference at all time points except for the time to endotracheal tube cuff inflation in three different airway simulations using personal protective equipment and an aerosol box (p<0.05). The median intubation times were longer using PPE and an AB. Conclusion: The use of personal protective equipment and an aerosol box significantly increases the total intubation time. Keywords: Endotracheal intubation, Personal protective equipment, Aerosol box, Video-laryngoscope