Among all the limiting factors in plant growth, soil salinity is one of the critical factors, but mechanisms of its effects in Spinacea oleracea especially the compartmentalization of Na+ and K+ need further investigations. In this study different concentrations of Nacl to S. oleracea . The objective was to study the growth, anatomy, physiology and non-structural carbohydrates. The other objective was to investigate Na+ and K+ compartmentalization in different plant tissues. We found that application of sodium significantly reduced the studied parameters of growth, anatomy, and physiology. Maximum amount of Na+ was compartmentalized in leaf-blade while the minimum in roots. Similarly, maximum K+ was compartmentalized in mid-rib and minimum in stem. The Na+: K+ decreased significantly with the increasing salinity application. Non-structural carbohydrates also decreased. Further, some significant relationships were also found between Na+, K+ and other traits. The study concludes that S. oleracea can tolerate salinity stress up to 80 mML-1. The findings can be helpful to cultivate S. oleracea in saline areas and can also be useful to cultivate other crops when successive cultivation of S. oleracea is carried out and Na+ is absorbed by crop from soil.