Study design and participants
This study presents a retrospective evaluation of the 238 patients admitted to Health Sciences University Samsun Training and Research Hospital who applied with suspicion of COVID-19 between March 11 and May 30, 2020. PCR results are determined in two categories, PCR positive group, and PCR negative group in line with The World Health Organization’s guide titled ”Laboratory Testing for 2019 Novel Coronavirus (2019-nCoV) in Suspected Human Cases” published on March 2, 2020 and TC Ministry of Health COVID-19 Guidelines.
All samples were studied with the SARS CoV-2 Double Gene RT-q PCR Kit (BioSpeedy, Turkey) in accordance with the manufacturer’s instructions. Briefly, after nucleic acid isolation in nasopharyngeal lavage/aspirate, broncho alveolar lavage, nasopharyngeal suture, oropharyngeal swab and sputum samples, detection by single-step reverse transcription (RT) and Real-Time PCR targeting the ORF1ab and N gene regions were performed.
Extract ion and inhibition control were checked by targeting the human RNase P gene as an internal control. Nucleic acid extraction was validated with the vNAT buffer and this process was conducted without any additional work during sample transfer. Nasopharyngeal swap ororopharyngeal swab samples taken with swabs (dacronor polyester flock) were placed in a sterile transport solution containing vNAT solution and transferred. Reaction components 2X Prime Script mix 10µL, CVD Di Oligo mix 5µL, template nucleic acid 5µL total reaction amount of 20µLvolume was created. Qiagen Rotor Gene (Germany) Real-Time PCR instrument was run on sigmoidal curves under 38 cycles and were evaluated as positive.
Prothrombin time (PT), activated partial thromboplastin time (aPTT), D-dimer, International Normalized Ratio (INR) and platelet (PLT) levels of 119 patients followed in COVID-19 services were taken from hospital information management system. In addition, PT, aPTT, D-dimer, INR and PLT levels of 119 individuals with COVID-19 PCR negative were taken.
D-dimer test was worked on a Beckman Coulter Au 680 device, PT, aPTT and INR tests were worked at Siemens Ca-7000 device and platelet level was worked in Beckman Coulter Dx-800 device by using suitable tubes and kits.