In the present study, the effect of different nitrogen sources on the anaerobic digestion of cheese whey was tested in batch scale reactors concerning methane production, free ammonia, and hydrogen sulfide. The results showed that supplementation with urea at a concentration of 1000 mg L-1 led to less variation in the FOS/TAC ratio, permitting greater stability to the anaerobic digestion process and as well as having the highest methane production values of 513.95 ± 2.12 mL CH4 g VS-1. On the other hand, supplementation with ammonium nitrate at a concentration of 1000 mg L-1 gave a lower value of methane 415.93 ± 5.44 mL CH4 g VS-1 and exhibited the lowest value of hydrogen sulfide 267.69 ± 0.37 ppm, as well as recorded the lowest free ammonium concentration of 49.18 ± 9.66 mg L-1. The findings from this research contributed to elucidate the role of urea, ammonium sulfate, and ammonium nitrate in the anaerobic digestion process, which could help to solve some problems related to the reduction in biogas and methane yield, correlated to the presence of hydrogen sulfide and free ammonium in cheese whey fed biodigesters.