The genetic structure of parasites is expected to mirror that of their hosts, as host dispersal is the primary driver to parasite spread. Theileria ( T.) annulata is a tick-borne protozoan parasite that is responsible for bovine theileriosis worldwide. The present study examined analyzed a set of specific micro/mini-satellites and the genetic structure of T. annulata in cattle breeds from Pakistan (Indian sub-continent) compared to that in Oman (Middle East), Tunisia (Africa) and Turkey (Europe). A high level of genetic diversity was observed among T. annulata detected in cattle from Pakistan comparable to that in Oman, Tunisia, and Turkey. T. annulata genotypes from these four countries form genetically distinct groups that are geographically sub-structured and this trend is correlated with geographical distance between these countries. The parasite population in Oman overlapped with that the T. annulata population in Africa and the Indian Subcontinent. The observed pattern of genetic differentiation of T. annulata accords well with that seen among cattle breeds in the Near East - European (Turkey), African (Libya), the Indian-Subcontinent (India) and the Arabian Peninsula. In conclusion, we are reporting dispersal of host affects the genetic structure of T. annulata.