2.1 DNA sampling and tissue collections
A female adult (C. sonnerati ), bred at the farm of Hainan,
Dongfang, Gancheng, China, was used for genome sequencing and assembly.
The fish was dissected immediately after treatment with 0.2 M eugenol.
Genomic DNA of C. sonnerati was collected from the caudal vein by
a Qiagen Blood & Cell Culture DNA Midi Kit, which was used for genome
sequencing. The muscle tissue was used for Hi-C library construction in
order to obtain a chromosome-scale genome assembly. Furthermore, tissues
from the liver, gill, intestines, kidney, head kidney, brain, pituitary,
gonad, heart, skin, and muscle were collected and quickly frozen in
liquid nitrogen before RNA sequencing, and then the tissues were kept at
–80°C at Sun Yat-Sen University.