Abstract
The
tomato hindCephalopholis
sonnerati (Valenciennes) (Serranidae), belonging to the genusCephalopholis , is a bottom dwelling coral reef of 12–120-m depth
in the Indo-Pacific and Red Sea. C. sonnerati has also
been characterized by complex social structures and behavioural
mechanisms. However, due to the lack of genomic resource forC. sonnerati ,
molecular-genetic studies and genomic breeding remain unexplored in this
species.
In
this study, we reported the chromosome-level genome assembly ofC.sonnerati using PacBio sequencing and Hi-C
sequencing technologies.
We obtained a total length of
1043.66 Mb with an N50 length of 2.49 Mb,
containing
795 contigs assembled into 24 chromosomes. Overall 95.8% of the
complete BUSCOs were identified in the assembled genome, suggesting the
completeness of the genome. Then, we predicted 26,130 protein-coding
genes, of which 94.26% were functionally annotated. In addition,C. sonnerati diverged from its common ancestor withE. lanceolatus and E. akaara approximately 41.7 million
years ago. Finally, we found tissue-specific expression of 8,108 genes.
Functional analyses showed that they mainly consisted of complement and
coagulation cascades, DNA replication, synaptic vesicle cycle, long-term
potentiation and other glycan degradation. Furthermore,
comparative genome analyses
indicated that the expanded genes families were highly enriched in the
sensory system, which was different from the enrichment analysis of the
tissue-specific expression genes. In brief, to our knowledge, we
reported the first
chromosome-level
genome assembly of C. sonnerati , which will provide a
valuable genome resource for studies on the genetic conservation,
resistance breeding, and evolutionary of C. sonnerati .