3.3 Phylogeny and divergent time
We identified 698 single-copy orthologues by using the sequencing similarities among protein-coding genes between 15 selected species. Additionally, a phylogenetic tree was constructed on the 678 filtered single-copy orthologues from 15 species genomes to reveal the phylogenetic relationship between them. We found that the C. sonneratidiverged approximately 41.7 million years ago (mya) from the common ancestor withE. lanceolatus and E. akaara . In addition,P.leopardus was the most closely related ancestor species to theC. sonnerati , separating from their common ancestor 66.4 to 75.7 mya (Figure 4).