A survey was conducted in 2019 – 2020, to study the disease incidence in major chilli growing areas of Andhra Pradesh and Tamil Nadu and also to study the cultural, morphological and pathogenicity of different isolates of Colletotrichum spp. The disease severity ranged from 8.13% to 38.66%. The highest disease incidence was recorded from Ramarkoodal village of Dharmapuri district in Tamil Nadu and lowest disease incidence was recorded from Palur village of Cuddalore district in Tamil Nadu. In pathogenicity test among all the isolates, CC 7 from Kamapatti exhibited maximum disease incidence (fruit rot incidence) and was found to be more virulent.