Aim: In this study, we aimed to investigate anxiety, depression, and sleep problems in healthcare professionals during the pandemic. Materials and Methods: This descriptive and cross-sectional study was conducted with Diyarbakır Gazi Yaşargil Training and Research Hospital employees. A total of 170 people were reached for the research. The data collected by the on-line survey method consisted of demographic questions, questions about behavior change due to COVID-19, Generalized Anxiety Disorder Scale (GAD-7), Patient Health Questionnaire (PHQ-9), and Insomnia Severity Index (ISI). Results: It was found that 72.9% of the healthcare professionals had anxiety, 83.5% had depression, and 87.1% had sleep problems. The total score of the GAD-7 scale was found to be significantly higher in those who used personal protective equipment (p=0.021) and those who received infection training (p<0.001). The PHQ-9 scale total score was higher in those 35 years of age and older (p=0.019) and college graduates (p=0.023). The total score of the insomnia severity scale (ISI) was significantly higher in those 35 years of age and older (p=0.040), in college graduates (p=0.049), and single/divorced (p=0.009). A significant difference was found between gender, occupation, smoking, fear of infection, burnout status, and total scores of all three scales (p<0.05). Conclusion: Healthcare professionals were found to have high levels of anxiety, depression, and sleep problems. Anxiety, depression, and insomnia problems were higher in women, health technicians, smokers, those with fear of infection, and those with burnout.