Abstract Objective: Live-based exercise programs offer great potential for the implementation of evidence-based interventions focused on promoting healthy habits. The outbreak of COVID-19 provided an opportunity to undertake an online survey to study the changes in exercise barriers/benefits, fatigue level, and quality of life after online live exercise program during lockdown, or quarantine. Methods: The sample was composed of a total of 143 women (age Mean = 32.48±6.6yrs) from a convenience sample of participants was recruited through social media (e.g. Facebook, Twitter, WhatsApp). The questionnaire asked for demographic information, body weight, body height, physical activity, and lifestyle factors before and during the quarantine. One-hundred and forty-three women volunteers were received an online live exercise program including strengthening, HIIT and stretching 6 sessions in a week during COVID19 pandemic and were assessed the measures of recruitment, exercise benefits/barriers, fatigue severity level and general quality of life at baseline, 3rd-wk and 6th-wk of the exercise program. Results: Significant differences were observed in fatigue severity scale and health status between baseline and at 3rd-wk (p < 0.05) in women. No difference in the level of fatigue, barriers, benefits of exercise, quality of life and health status between baseline and at 3rd-wk, and 3rd-wk and 6th-wk or baseline and 6th-wk (p>0.05). Conclusion: This study demonstrates that the internet-based exercise program is a viable alternative for the delivery and dissemination of interventions focused on promoting healthy habits, and a totally self-administered intervention can also produce short-term positive results. This program supports during lockdown reduce the risk of developing persistent fatigue related chronic fatigue syndrome.