2.5 Double luciferase assay
The circRNAs were built into PSI‐CHECK2 vector, then circRNA and miRNA
were cotransfected into 293T cells.Luciferase activities were measured
at 48‐hours after transfection using the Dual‐Luciferase Reporter
AssaySystem (Promega, Madison, WI) according to themanufacturer’s
protocol.Renilla luciferase activity wasnormalized to firefly luciferase
activity and expressed as apercentage of the control. All transfection
experiments were performed in triplicate.