2.8 Cell culture was transfected with SiRNA.
TLR2SiRNA,TLR4SiRNA and TLR8SiRNA were purchased from Huzhou Hippo
Biotechnology Co., LTD, the specific sequence is shown in S1. 6cm cell
dishes were taken and 2mL (1~2×105cells) culture medium was added into each well. The culture density
reached 70-90% at 37℃ and 5%CO2; Dilute Lipofectamine
3000 (Invitrogen, No.3000015) with Opti-MEM and mix well; Dilute the
carrier with Opti-MEM, add P3000, and mix well; Diluted carrier (1:1)
was added into the diluted Lipofectamine 3000 and incubated at room
temperature for 5 minutes; Add the mixture into the culture medium,
shake well and incubate at 37℃ for 3 days.