During our ongoing efforts to explore macrofungal diversity of Pakistan, two new records i.e. Collybiopsis rodhallii and Marasmius trichotus were collected and identified using morphological and molecular characterization. Their detailed description and illustration are provided. In addition, phylogenetic analysis using nrITS data also support the identity of these new records. Previously, only three species of Collybiopsis and thirteen Marasmius have been described from Pakistan. Moreover, Paramarasmius palmivours which has already been reported from Pakistan is now recorded from a different locality district Mandi Bahauddin of Pakistan during this study. The aim of this study is to accurately identify saprobic fungi of Pakistan using morphological and molecular tool and contribute towards exploration of fungal diversity of Pakistan.