Figure Legends
Figure 1. Heat stroke decreased the expression of HSPG and concentration of SDC-1 and HS in HPMEC. (A). Representative immunofluorescence images of HSPG in glycocalyx from each groups at magnification × 100. Green indicated HSPG, and blue indicated nuclear. (B-C). HSPG concentration in each group was detected using flow cytometry. (D). Concentration of SDC-1 and HS in cell culture supernatant in HPMEC from each group. *P< 0.05 versus CON, #P < 0.05 versus HS; n = 8.
Figure 2. Heat stroke induced cells apoptosis in HPMEC. (A) Representative morphological images of distinct groups in HPMEC using TEM. (B) The apoptotic rates in each group were measured by flow cytometry. (C). Quantitative analysis of the apoptotic rates in distinct groups. *P < 0.05 versus CON,#P < 0.05 versus HS; n = 8.
Figure 3. Heat stroke changed expression of coagulation enhancers (vWF and ET-1), adhesion proteins, and tight proteins. (A). Concentration of vWF and ET-1 were performed using ELISA. (B). Western blot were performed to detect expression of E-selectin, VCAM-1, and occludin, and quantification of the proteins. The data are presented as mean values ± SEM for independent experiments.*P < 0.05 versus CON,#P < 0.05 versus HS; n = 8.
Figure 4. Heat stroke exacerbated the inflammatory state and oxidative stress levels. (A). ELISA was performed to detect concentration of TNF and IL-6. (B). The level of intracellular ROS was measured with a DCFH-DA. *P < 0.05 versus CON, #P < 0.05 versus HS; n = 8.