Background: The COVID-19 pandemic caused by the SARS-CoV-2 virus is still causing extensive sickness, death, and social and economic devastation worldwide. Effective prophylactic strategies such as personal protection measures, social distancing, and vaccination is critical in limiting disease transmission. However, more than two years after the emergence of COVID-19, the pandemic continues to have a global impact, with new variants and surges in cases and fatalities. Objectives: This study aimed to investigate the Pakistani public’s perception and knowledge regarding various aspects of COVID-19 after a period of two years of the pandemic. The study also aimed to investigate the association between education level and knowledge about COVID-19. Methods: An online cross-sectional survey was conducted using a convenience sample of 1100 individuals from different cities in Pakistan. The survey consisted of various sets of questions covering the aspects of the study objectives. Results: Most participants reported a solid understanding of COVID-19; however, there were some misconceptions and knowledge gaps concerning certain pandemic elements. The study also highlighted a link between education level and awareness of COVID-19, with a higher education level related to a better understanding of the pandemic and effective preventive strategies. Conclusion: This study provides valuable insight into the perception and education level of the Pakistani public regarding COVID-19. The findings highlight the need of continued public health efforts to promote and ensure access to effective prevention measures, particularly in vulnerable populations who may face barriers to obtaining protective equipment.