Serum biochemistry
About two ml of blood was collected 1 hour before and 5 min after
exercise in a clot activator vacutainer and kept in a slanting position
until the clotting occurred. Serum was separated using Remi R-8C
centrifuge machine at a speed of 2500 rpm for three minutes (Hassanet al., 2015). Quantitative estimation of parameters like
Glucose, Cholesterol, Triglycerides, Total Protein, Albumin, Aspartate
Aminotransferase (AST), Alkaline Phosphatase (ALP), Blood Urea Nitrogen,
Creatinine, Calcium, Phosphorus, Magnesium, Creatine Kinase-MB (CK-MB)
and Lactate dehydrogenase (LDH) were carried out using fully automated
biochemical analyzer (A15 Biosystem Random Access Analyzer, Spain) with
specific diagnostic kits following the manufacturer’s protocol. Sodium,
Potassium, Chloride was estimated in an automated electrolyte analyzer
(Diestro 103 AP, Argentina).