Materials and methods
The healthy Indian Kathiawari horses that were brought for periodical health checkups to the Large Animal Medicine Out-patient Unit of Madras Veterinary College Teaching Hospital were subjected to this clinical study. Indian Kathiawari horses regardless of sex, breed and age were selected for the study and subjected to thorough physical examination (Allaam et al., 2014) , to confirm the health condition of horses. Eighteen working Kathiawari horses of age ranging from 2 years to 12 years consisting of 10 stallions and 8 mares were selected for this study. Consent for exercise was obtained from all the horse owners. Horses were subjected to exercise for a period of about 45 mins (Rossi et al., 2019) . 2 mL of whole blood and serum samples were collected 1 hour before and 5 min after exercise for evaluation of changes in haematobiochemical concentration.