Study design
The healthy horses that were brought for periodical health checkups to the Large Animal Medicine Out-patient Unit of Madras Veterinary College Teaching Hospital were subjected to this clinical study. Indian Kathiawari horses regardless of sex, breed and age were selected for the study and subjected to thorough physical examination (Allaamet al., 2014) , electrocardiography and echocardiography (two-dimensional, M mode and color Doppler) to confirm the health condition of horses. Eighteen working Kathiawari horses of average age 6.19 ± 0.75 ranging from 2 years to 12 years and body weight 332.28 ± 15.41 ranging from (195 Kg to 430 Kg) and consisting of 8 mares and 10 stallions were selected. Their hemato-biochemical assessments were carried out. Consent for exercise was obtained from all the horse owners. Horses were subjected to exercise for a period of ± 40 mins (Rossi et al., 2019) . 2 mL of Serum samples were collected 1 hour before and 3 hour after exercise for evaluation of changes in cardiac biomarkers concentration. Electrocardiographic and echocardiographic evaluations were also done for same horses 1 hour before and 5min after exercise.