Cardiac biomarkers
Two mL blood was collected 1 hour before and 3hours after exercise in a
clot activator vacutainer and allowed to stand for some time until the
clotting occurred and the serum samples was separated. Cardiac
biomarkers such as Cardiac Troponin T, Cardiac Troponin I and NT-pro BNP
were analyzed (Hammerer-Lercher, 2001) using fully automatic
fluorescence based hormone analyzer (sandwich immunodetection method/
iCHROMA III).