Cardiac biomarkers
Two mL blood was collected 1 hour before and 3hours after exercise in a clot activator vacutainer and allowed to stand for some time until the clotting occurred and the serum samples was separated. Cardiac biomarkers such as Cardiac Troponin T, Cardiac Troponin I and NT-pro BNP were analyzed (Hammerer-Lercher, 2001) using fully automatic fluorescence based hormone analyzer (sandwich immunodetection method/ iCHROMA III).