Collection data and preparation of samples
The demographic and general characteristics of the patients and their
lesions were collected from clinical history.
Extralesional and intralesional venous blood samples were obtained from
patients in 3.8% sodium citrate tubes (Vacutainer®,
BD, Becton Dickinson and Company, Madrid, Spain). Platelet-poor plasma
(PPP) was obtained by centrifuging the whole blood at 2500 g for 15 min
at 23ºC. Plasma samples were stored at -80ºC until analysis.