Gross Necropsy and Histology
Deeply sedated swine were perfused intravenously with 1% TTC, then
euthanized with an intravenous bolus of 130-150mg/mL saturated potassium
chloride. Hearts were explanted, examined, and photographed. Ventricular
lesions were cross-sectioned and individually assessed and measured by a
blinded operator using a digital microscope (Dino-Lite Edge, AnMo
Electronics). Ventricular lesions and atrial lesion sets were formalin
fixed and processed for histological evaluation with hematoxylin and
eosin (H&E) and Masson’s Trichrome stains.