Gross Necropsy and Histology
Deeply sedated swine were perfused intravenously with 1% TTC, then euthanized with an intravenous bolus of 130-150mg/mL saturated potassium chloride. Hearts were explanted, examined, and photographed. Ventricular lesions were cross-sectioned and individually assessed and measured by a blinded operator using a digital microscope (Dino-Lite Edge, AnMo Electronics). Ventricular lesions and atrial lesion sets were formalin fixed and processed for histological evaluation with hematoxylin and eosin (H&E) and Masson’s Trichrome stains.