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Iron uptake by Escherichia coli in urinary tract infections and urosepsis -- genetic and proteomic studies
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  • Beata Krawczyk,
  • Magdalena Burzyńska,
  • Paweł Wityk,
  • Agnieszka Laskowska,
  • Anna Stanisławska-Sachadyn,
  • Joanna Raczak-Gutknecht,
  • Małgorzata Waszczuk-Jankowska,
  • Marek Bronk,
  • Michał J. Markuszewski
Beata Krawczyk
Politechnika Gdanska

Corresponding Author:beata.krawczyk@pg.edu.pl

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Magdalena Burzyńska
Politechnika Gdanska
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Paweł Wityk
Politechnika Gdanska
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Agnieszka Laskowska
Politechnika Gdanska
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Anna Stanisławska-Sachadyn
Politechnika Gdanska
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Joanna Raczak-Gutknecht
Gdanski Uniwersytet Medyczny Katedra Biofarmacji i Farmakodynamiki
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Małgorzata Waszczuk-Jankowska
Gdanski Uniwersytet Medyczny Katedra Biofarmacji i Farmakodynamiki
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Marek Bronk
Gdanski Uniwersytet Medyczny
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Michał J. Markuszewski
Gdanski Uniwersytet Medyczny Katedra Biofarmacji i Farmakodynamiki
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Abstract

Urinary tract infection caused by Escherichia coli can lead to urosepsis, and the pathogens’ effectiveness in acquiring iron determines their virulence. The level of expression of the siderophore genes in different environments (M9 medium, artificial urine, and M9 supplemented with blood) was investigated using the CAS assay and qPCR. Aerobactin was found much more frequently in blood than in urine isolates (P=0.03), but the expression in artificial urine was 2x higher than in blood (P=0.03). We observed significant changes in the expression of entC, iro-2, iucA, and iroB bacterial genes between groups in artificial urine. The siderophores enterobactin, aerobactin and yersiniabactin (P=0.016), with the iha and hlyA genes, may represent markers of increased urosepsis risk. Moreover, proteomic profiles between groups were significantly different (ferritin-1, iron uptake system component EfeO, ferrous iron transport protein B, nitrate/nitrite response regulator protein NarL, protein HemY, ferrienterobactin receptor FepA, lipopolysaccharide export system protein LptA and 2Fe-2S ferredoxin). We also observed the positive association with urosepsis of IL-6, TNF, and CRP proteins between groups. In conclusion, risk factors for UTI-related sepsis may be related to the iron uptake system, and genetic and proteomic profiles may help in their identification.
04 Nov 2023Submitted to Molecular Microbiology
04 Nov 2023Assigned to Editor
04 Nov 2023Submission Checks Completed
06 Nov 2023Reviewer(s) Assigned